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anti cd56 fitc  (Miltenyi Biotec)


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    Miltenyi Biotec anti cd56 fitc
    Anti Cd56 Fitc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd56+fitc/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm41975066-401-27-33
    Average 96 stars, based on 42 article reviews
    anti cd56 fitc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Optimization of hypo-alloimmunogenic multispecific CAR-T and SARS-CoV-2-specific T cells for off-the-shelf adoptive cell therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and FlowJo, respectively (BD Bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42) and human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M KO; Biotin-CD19 CAR Detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 extracellular domains; cat. 130-115-965) and Streptavidin-APC-Vio 770 (cat. 130-106-794) for CAR expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520), and CD19-PE-vio770 (REA675; cat. 130-113-647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557), and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi Biotec) for T cell subsets of SARS-CoV-2-specific T cells; CD3-FITC, HLA-ABC-PE, and HLA-E (3D12; cat. 342606, Biolegend) for knockin analysis; CD3-BV421 (UCHT1; cat. 300434, BioLegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi Biotec), HLA-ABC-PE, and HLA-E for NK92MI co-culture; CD3-APC/Cyanine7 (SK7; cat. 344818, BioLegend), CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565), and NKG2C-APC (REA205; cat. 130-117-547) (Miltenyi Biotec) for NK cells analysis. ..

    Article Title: Optimization of Hypo-alloimmunogenic Multispecific CAR-T Cells and SARS-CoV-2-Specific T Cells for Off-the-shelf Adoptive Cell Therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and 503 Flowjo respectively (BD bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42), 504 human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M knockout; biotin-CD19 505 CAR detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 506 extracellular domains; cat. 130-115-965) and streptavidin-APC-Vio® 770 (cat. 130-106-794) for CAR 507 expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-508 viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; 509 cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520) and CD19-PE-vio770 (REA675; cat. 130-113-510 647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557) 511 and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi biotec) for T cell subsets of SARS-CoV-2-512 specific T cells; CD3-FITC, HLA-ABC-PE and HLA-E (3D12; cat. 342606, Biolegend) for knock-in analysis; 513 CD3-BV421 (UCHT1; cat. 300434, Biolegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi biotec), 514 HLA-ABC-PE and HLA-E for NK92MI co-culture; CD3-APC/ Cyanine7 (SK7; cat. 344818, Biolegend), 515 CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565) and NKG2C-APC 516 (REA205; cat. 130-117-547) (Miltenyi biotec) for NK cells analysis. ..

    Article Title: The impact of anticoagulant used for peripheral blood collection on NK cell yield, function, and proliferation.
    Article Snippet: Natural killer (NK) cells are under investigation for cellular immunotherapy of cancer, infectious diseases, and autoimmunity.. The collection of autologous or allogeneic starting material for the expansion of NK cells whether whole blood, cord blood, or leukocyte apheresis requires the use of anticoagulants.. Heparin and acid citrate dextrose A (ACD-A) are most commonly used for collection, but their impact on NK cells has not been clearly described.

    Knock-In:

    Article Title: Optimization of hypo-alloimmunogenic multispecific CAR-T and SARS-CoV-2-specific T cells for off-the-shelf adoptive cell therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and FlowJo, respectively (BD Bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42) and human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M KO; Biotin-CD19 CAR Detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 extracellular domains; cat. 130-115-965) and Streptavidin-APC-Vio 770 (cat. 130-106-794) for CAR expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520), and CD19-PE-vio770 (REA675; cat. 130-113-647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557), and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi Biotec) for T cell subsets of SARS-CoV-2-specific T cells; CD3-FITC, HLA-ABC-PE, and HLA-E (3D12; cat. 342606, Biolegend) for knockin analysis; CD3-BV421 (UCHT1; cat. 300434, BioLegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi Biotec), HLA-ABC-PE, and HLA-E for NK92MI co-culture; CD3-APC/Cyanine7 (SK7; cat. 344818, BioLegend), CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565), and NKG2C-APC (REA205; cat. 130-117-547) (Miltenyi Biotec) for NK cells analysis. ..

    Article Title: Optimization of Hypo-alloimmunogenic Multispecific CAR-T Cells and SARS-CoV-2-Specific T Cells for Off-the-shelf Adoptive Cell Therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and 503 Flowjo respectively (BD bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42), 504 human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M knockout; biotin-CD19 505 CAR detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 506 extracellular domains; cat. 130-115-965) and streptavidin-APC-Vio® 770 (cat. 130-106-794) for CAR 507 expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-508 viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; 509 cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520) and CD19-PE-vio770 (REA675; cat. 130-113-510 647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557) 511 and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi biotec) for T cell subsets of SARS-CoV-2-512 specific T cells; CD3-FITC, HLA-ABC-PE and HLA-E (3D12; cat. 342606, Biolegend) for knock-in analysis; 513 CD3-BV421 (UCHT1; cat. 300434, Biolegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi biotec), 514 HLA-ABC-PE and HLA-E for NK92MI co-culture; CD3-APC/ Cyanine7 (SK7; cat. 344818, Biolegend), 515 CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565) and NKG2C-APC 516 (REA205; cat. 130-117-547) (Miltenyi biotec) for NK cells analysis. ..

    Staining:

    Article Title: HLA-G neo-expression modifies genetic programs governing tumor cell lines
    Article Snippet: .. The following antibodies were used for cell surface staining: MEM-G/9-PE (anti-HLA-G1) from Exbio (Praha, a.s.); ILT2-PE (Clone: HP-F1) and ILT4-PE from eBioscience (San Diego, CA, U.S.); CD147-PE, SDC2-APC; CD56-PE, CD56-FITC and HVEM-PE from Miltenyi Biotec (Bergisch Gladbach, North Rhine-Westphalia, Germany); KIR2DL4-PE; NRP1-PE from BioLegend (San Diego, CA, U.S.); CD160 from Abcam (Cambridge, U.K.) and CD160-PE from Beckman Coulter (Brea, CA, U.S.). ..

    Article Title: Interferon-gamma biased agonists
    Article Snippet: Human blood DCs were enriched from blood in leukoreduction system chambers by Ficoll-Hypaque density gradient centrifugation, followed by magnetic enrichment with the EasySep Human Myeloid DC Enrichment kit (19061; StemCell Technologies). .. Enriched cells were stained with DAPI and lineage markers CD19 PE-Cy5 (Beckman Coulter); CD56 FITC, CD3 Alexa700 (BioLegend); CD11c PE-Cy7, HLA-DR v500, CD14 APC-H7 (BD); and CD304 PE (MACs Miltenyi Biotec). ..

    Article Title: The impact of anticoagulant used for peripheral blood collection on NK cell yield, function, and proliferation.
    Article Snippet: Natural killer (NK) cells are under investigation for cellular immunotherapy of cancer, infectious diseases, and autoimmunity.. The collection of autologous or allogeneic starting material for the expansion of NK cells whether whole blood, cord blood, or leukocyte apheresis requires the use of anticoagulants.. Heparin and acid citrate dextrose A (ACD-A) are most commonly used for collection, but their impact on NK cells has not been clearly described.

    Knock-Out:

    Article Title: Optimization of Hypo-alloimmunogenic Multispecific CAR-T Cells and SARS-CoV-2-Specific T Cells for Off-the-shelf Adoptive Cell Therapy
    Article Snippet: Cells acquisition and analysis was performed by BD LSRII and 503 Flowjo respectively (BD bioscience). .. Antibodies to use: human CD45-APC (2D1; cat. 17-9459-42), 504 human HLA-ABC-PE (W6/32; cat. 12-9983-42) (both from eBioscience) for B2M knockout; biotin-CD19 505 CAR detection Reagent (which recombinantly expressed fusion protein consisting of the human CD19 506 extracellular domains; cat. 130-115-965) and streptavidin-APC-Vio® 770 (cat. 130-106-794) for CAR 507 expression; CD45-vioblue (5B1; cat. 130-113-122), CD3-FITC (REA613; cat. 130-113-138), CD4-508 viogreen (REA623; cat. 130-113-230), CD8-APC-vio770 (REA734; cat. 130-110-681), CD56-PE (REA196; 509 cat. 130-113-312), CD14-APC (REA599; cat. 130-110-520) and CD19-PE-vio770 (REA675; cat. 130-113-510 647) for immune composition; CD3-FITC (REA613), CD45RO-APC-vio770 (REA611; cat. 130-113-557) 511 and CD62L-PE (REA615; cat. 130-114-151) (all from Miltenyi biotec) for T cell subsets of SARS-CoV-2-512 specific T cells; CD3-FITC, HLA-ABC-PE and HLA-E (3D12; cat. 342606, Biolegend) for knock-in analysis; 513 CD3-BV421 (UCHT1; cat. 300434, Biolegend), CD56-FITC (REA196; cat. 130-113-312, Miltenyi biotec), 514 HLA-ABC-PE and HLA-E for NK92MI co-culture; CD3-APC/ Cyanine7 (SK7; cat. 344818, Biolegend), 515 CD56-PE (REA196; cat. 130-114-549), NKG2A-FITC (REA110; cat. 130-113-565) and NKG2C-APC 516 (REA205; cat. 130-117-547) (Miltenyi biotec) for NK cells analysis. ..

    Blocking Assay:

    Article Title: The impact of anticoagulant used for peripheral blood collection on NK cell yield, function, and proliferation.
    Article Snippet: Natural killer (NK) cells are under investigation for cellular immunotherapy of cancer, infectious diseases, and autoimmunity.. The collection of autologous or allogeneic starting material for the expansion of NK cells whether whole blood, cord blood, or leukocyte apheresis requires the use of anticoagulants.. Heparin and acid citrate dextrose A (ACD-A) are most commonly used for collection, but their impact on NK cells has not been clearly described.

    Saline:

    Article Title: The impact of anticoagulant used for peripheral blood collection on NK cell yield, function, and proliferation.
    Article Snippet: Natural killer (NK) cells are under investigation for cellular immunotherapy of cancer, infectious diseases, and autoimmunity.. The collection of autologous or allogeneic starting material for the expansion of NK cells whether whole blood, cord blood, or leukocyte apheresis requires the use of anticoagulants.. Heparin and acid citrate dextrose A (ACD-A) are most commonly used for collection, but their impact on NK cells has not been clearly described.

    Labeling:

    Article Title: HLA I immunopeptidome of synthetic long peptide pulsed human dendritic cells for therapeutic vaccine design.
    Article Snippet: Anti-human protein antibodies used for moDC analysis were: CD45APCeFluor780 (0.31 μg/mL; HI30, Thermo Fisher), CD14-eFluor450 npj Vaccines | (2025) 10:12 10 (2 μg/mL; 61D3, Thermo Fisher), CD1a-APC (0.15 μg/mL; HI149, BD Pharmingen), DC-SIGN-PerCP-Cy5.5 (0.08 μg/mL; DCN46, BD Pharmingen), CD11c-FITC (5 μg/mL; KB90, Dako), CD80-FITC (5 μg/mL; MAB104, Thermo Fisher), CD83-APC (0.67 μg/mL; HB15e, Thermo Fisher), HLA-DR-PE (0.02 μg/mL; LN3, Thermo Fisher), CD40-PerCPeFluor710 (0.08 μg/mL; 5C3, Thermo Fisher), and the viability dye AQUA Live/Dead-AmCyan (1:200; Invitrogen). .. Anti-human protein antibodies used for primary DC analysis were: CD45-eFluor450 (0.625 μg/mL, HI30, Thermo Fisher), CD19-FITC (2 μg/mL, HIB19, Thermo Fisher), CD3FITC (1.25 μg/mL, UCHT1, Thermo Fisher), CD14-FITC (2 μg/mL, 61D3, Thermo Fisher), CD56-FITC (0.03 μg/mL, NCAM16.2, BD Biosciences), CD11c-PE-Cy7 (4 μg/mL, 3.9, Thermo Fisher), HLA-DR-PerCP-Cy5.5 (0.06 μg/mL, LN3, Thermo Fisher), BDCA1-APC (1 recommended test volume, AD5-8E7,Miltenyi Biotech), BDCA2-APC (1:10, AC144,Miltenyi Biotech), BDCA3-APC (1.65 μg/mL, AD5-14H12, Miltenyi Biotech), and FcBlock (2.5 μg/mL,BDPharmingen).Cellswerewashedwith200 μLblock buffer after labeling andmeasuredon theBDFACSCanto (BDBiosciences). .. Analysis was performed using FlowJo v10.8.1 software (FlowJo).



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    Image Search Results


    Immunophenotypic characterization of NK cells isolated by natural migration and used in functional assays. Primary NK cells were enriched from peripheral blood mononuclear cells (PBMCs) using a natural migration-based selection approach and maintained in IL-15 supplemented medium prior to downstream assays. Immunofluorescence staining was performed to validate lineage identity and maturation status. (A) CD3 staining shows absence of detectable signal, excluding T-lymphocyte contamination. (B) CD56 expression identifies NK lineage cells within the enriched population. (C) CD16 positivity demonstrates the presence of cytotoxic NK subsets associated with antibody-dependent and direct target cell killing. (D) CD57 staining identifies a subset of NK cells exhibiting features consistent with functional maturation. Images were acquired under identical exposure settings for all markers. Scale bar = 100 μm. Collectively, these findings indicate that the enriched effector population consisted predominantly of CD3 − CD56 + NK cells with detectable CD16 and CD57 expression. Precise discrimination between CD56 bright and CD56 dim subsets requires flow cytometric analysis and was therefore not inferred from fluorescence intensity in these images.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Natural killer cell activated by attenuated newcastle disease virus (NDV) as anti-cancer therapy

    doi: 10.3389/fmolb.2026.1721060

    Figure Lengend Snippet: Immunophenotypic characterization of NK cells isolated by natural migration and used in functional assays. Primary NK cells were enriched from peripheral blood mononuclear cells (PBMCs) using a natural migration-based selection approach and maintained in IL-15 supplemented medium prior to downstream assays. Immunofluorescence staining was performed to validate lineage identity and maturation status. (A) CD3 staining shows absence of detectable signal, excluding T-lymphocyte contamination. (B) CD56 expression identifies NK lineage cells within the enriched population. (C) CD16 positivity demonstrates the presence of cytotoxic NK subsets associated with antibody-dependent and direct target cell killing. (D) CD57 staining identifies a subset of NK cells exhibiting features consistent with functional maturation. Images were acquired under identical exposure settings for all markers. Scale bar = 100 μm. Collectively, these findings indicate that the enriched effector population consisted predominantly of CD3 − CD56 + NK cells with detectable CD16 and CD57 expression. Precise discrimination between CD56 bright and CD56 dim subsets requires flow cytometric analysis and was therefore not inferred from fluorescence intensity in these images.

    Article Snippet: Chambers were incubated for 1 h at room temperature with FITC-conjugated mouse anti-human CD56 monoclonal antibody (Elabscience, China) diluted 1:100 in blocking buffer.

    Techniques: Isolation, Migration, Functional Assay, Selection, Immunofluorescence, Staining, Expressing, Fluorescence

    CD56 immunofluorescence demonstrates enhanced NK attachment to NDV-infected tumor cells. Representative immunofluorescence micrographs of AMJ13 (A–D) and MCF-7 (E–H) cells stained for CD56 (green) under the indicated treatment conditions. (A,E) Untreated tumor cells show negligible CD56 staining, confirming absence of NK cells. (B,F) NDV alone (MOI 20) does not produce CD56 signal, confirming antibody specificity. (C,G) NK cells alone (2000 cells/well) display modest CD56-positive signal following washing, indicating limited NK retention on tumor monolayers. (D,H) Combined NDV (MOI 20) + NK cells (2000 cells/well) markedly increases CD56 fluorescence, with visible clusters of CD56 + NK cells remaining attached to tumor cell surfaces after washing. Scale bar = 100 μm. (I) Quantitative analysis of retained CD56 + NK cells per microscopic field demonstrates significantly increased wash-resistant NK attachment following NDV priming. AMJ13 cultures exhibited greater than 10-fold enrichment of retained NK cells compared with NK-only treatment (**p < 0.01), while MCF-7 cultures showed approximately two-fold increased NK retention (*p < 0.05). Data represent mean ± SD derived from three independent microscopic fields per condition from separate wells. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Natural killer cell activated by attenuated newcastle disease virus (NDV) as anti-cancer therapy

    doi: 10.3389/fmolb.2026.1721060

    Figure Lengend Snippet: CD56 immunofluorescence demonstrates enhanced NK attachment to NDV-infected tumor cells. Representative immunofluorescence micrographs of AMJ13 (A–D) and MCF-7 (E–H) cells stained for CD56 (green) under the indicated treatment conditions. (A,E) Untreated tumor cells show negligible CD56 staining, confirming absence of NK cells. (B,F) NDV alone (MOI 20) does not produce CD56 signal, confirming antibody specificity. (C,G) NK cells alone (2000 cells/well) display modest CD56-positive signal following washing, indicating limited NK retention on tumor monolayers. (D,H) Combined NDV (MOI 20) + NK cells (2000 cells/well) markedly increases CD56 fluorescence, with visible clusters of CD56 + NK cells remaining attached to tumor cell surfaces after washing. Scale bar = 100 μm. (I) Quantitative analysis of retained CD56 + NK cells per microscopic field demonstrates significantly increased wash-resistant NK attachment following NDV priming. AMJ13 cultures exhibited greater than 10-fold enrichment of retained NK cells compared with NK-only treatment (**p < 0.01), while MCF-7 cultures showed approximately two-fold increased NK retention (*p < 0.05). Data represent mean ± SD derived from three independent microscopic fields per condition from separate wells. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test.

    Article Snippet: Chambers were incubated for 1 h at room temperature with FITC-conjugated mouse anti-human CD56 monoclonal antibody (Elabscience, China) diluted 1:100 in blocking buffer.

    Techniques: Immunofluorescence, Infection, Staining, Fluorescence, Derivative Assay